CUSTOM ITEM: HPLC Column, Bidentate C8, 4um, 3.0mm ID x 250mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑25P‑3 Cogent Bidentate C8™ HPLC Column is a custom‑manufactured, extended‑length mid‑bore analytical column designed for separations requiring maximum resolving power while significantly reducing solvent consumption compared to standard 4.6 mm ID formats. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, faster equilibration, and extremely robust column lifetime under demanding analytical conditions.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This non‑traditional chemistry produces a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, making it ideal when C18 retention is excessive but stronger interaction than standard C8 is still required. Unlike traditional silica‑based C8 phases, this column can also retain select hydrophilic compounds, offering expanded selectivity and method flexibility.
The 3.0 mm internal diameter supports lower flow rates and reduced solvent consumption, while the extended 250 mm length provides the highest plate count and resolving power in the standard Bidentate C8™ lineup. This combination enables separation of closely eluting or structurally similar compounds while maintaining run times comparable to larger‑ID columns.
Importantly, the solvent‑saving benefits of the 3.0 mm format are achieved without requiring specialized low‑flow instrumentation, allowing easy integration into standard analytical HPLC systems.
The column is also stable at very low pH (below pH 2) without degradation, enabling aggressive methods not feasible with conventional silica‑based C8 columns.
Why Choose the 3.0 × 250 mm Bidentate C8™ Format
This configuration is typically selected when:
Maximum analytical resolution is required
Shorter columns (50–150 mm) do not adequately separate critical pairs
C18 columns are overly retentive or slow
Solvent‑use reduction is desired without changing instrumentation
Complex mixtures require extended retention and separation space
The combination of long column length and mid‑bore geometry delivers peak resolution while maintaining operational efficiency.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase surface chemistry
Reduced silanol activity and secondary interactions
Faster equilibration than conventional silica‑based C8 columns
Extremely robust and reproducible performance
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Alternative selectivity when C18 is overly retentive
Maximum‑Resolution Mid‑Bore Geometry
3.0 mm ID × 250 mm length
Highest resolution among standard Bidentate C8™ formats
Lower solvent consumption than 4.6 mm ID columns
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Suitable for aggressive and unconventional analytical methods
Typical Applications
High‑resolution analytical HPLC
Complex or closely related compound separations
Alternative reversed‑phase selectivity vs. C18
Method development and validation
Low‑pH analytical methods
Solvent‑reduction and cost‑efficiency workflows
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable